ChIP-Seq Peak Calling and Data Analysis in Woody Plants
DOI:
https://doi.org/10.53555/nnas.v5i12.576Abstract
Chromatin immunoprecipitation (ChIP) followed by highthroughput sequencing (ChIP-Seq) provides an important tool to study genome-wide protein-DNA interactions to help understand gene regulation in the context of native chromatin. A complete ChIP-Seq workflow including peak calling, peak classification, transcription factor binding site overrepresentation and definition of new TFBS motifs. In this paper, ChIP-Seq peak calling and data analysis is described in detail.
References
Bolger A M , Lohse M , Usadel B . Trimmomatic: a flexible trimmer for Illumin a sequence data[J]. Bioinformatics, 2014, 30 (15):2114-2120.
Gupta S , Stamatoyannopoulos J A , Bailey T L , et al. Quantifying similarity between motif s[J]. Genome Biology, 2007, 8(2):R24-R24.
J. Ye , Coulouris G , Zaretskaya I , et al. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction[J]. BMC Bioinformatics, 2012, 13(1):134.
Langmead B, Salzberg S. Fast gapped-read alignment with Bowtie 2. Nature Meth ods. 2012, 9:357-359.
Martin R C , Vining K , Dombrowski J E . Genome-wide (ChIP-seq) identification of target genes regulated by BdbZIP10 during paraquat-induced oxidative stress[J]. Bmc Plant Biology, 2018, 18(1):58.
Martin M . Cutadapt removes adapter sequences from high-throughput sequencing read[J]. Embnet Journal, 2011, 17(1). 2114–2120.
Planet E , Attolini S O , Reina O , et al. htSeqTools: high-throughput sequencing quality control, processing and visualization in R[J]. Bioinformatics, 2012, 28(4):589-590.
T.L. Bailey, DREME: motif discovery in transcription factor ChIP-Seq data,Bioinformatics 27 (2011) 1653e1659.
Timothy L. Bailey, Mikael Bodén, Fabian A. Buske, Martin Frith, Charles E. Grant, Luca Clementi, Jingyuan Ren, Wilfred W. Li, William S. Noble, "MEME SUITE:tools for motif discovery and searching", Nucleic Acids Research, 37:W202-W208, 2009.
T.L. Bailey, C. Elkan, Fitting a mixture model by expectation maximization to discover motifs in biopolymers, Proc. Int. Conf. Intell. Syst. Mol. Biol. 2 (1994) 28-36.
Wang S, Sun H, Ma J, et al. Target analysis by integration of transcriptome and ChIP-Seq data with BETA[J]. Nature Protocols, 2013, 8(12):2502-25.
Zhang Y , Liu T , Meyer C A, et al. Model-based analysis of chip-seq (MACS) [J]. Genome biology, 2008, 9(9):R137.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Terms & Condition
Submission -
Author can submit the manuscript through our online submission process or email us at the designated email id in contact details.
The other mode of submission not accepted than online and email.
Before submission please read the submission guidelines.
NN Publication accepts only article submitted in pdf/doc/docx/rtf file format. Another format except given file formats will no be considered .
Author will be responsible for the error mistakes in the submission files. The minor changes can be done without any cost after publication. But for major changes NN Publication may charges you the editing charges.
Publication (Online) -
The online publication is scheduled on last date of every month, but it can be delayed by 24 to 48 hours due to editorial process if huge number of articles comes to publish in single issue.
Automatic notificatation email will be sent to the all users on publication of an issue, so its author’s duty to check their email inbox or SPAM folder to get this notification.
After publication of article author can not withdraw their article.
If editor’s found any issue after publication of article then the NN Publication have the authority to remove the article from online website.
No refund will be provided after online publication of article.
Publication (Print) -
The print copy publication are sent as per the author’s request after 2 weeks of online publication of that issue.
NN Publication will ship the article by India Post and provide the consignment number on dispatch of print copy.
NN Publication follows all the guidelines of delivery provided by IndiaPost and hence not responsible for delay in delivery due to any kind of reasons.
Refund of hard copy will not be provided after dispatch or print of the journal.
NN Publication will be responsible for raise a complain if there is any issue occurs in delivery, but still will not be responsible for providing the refund.
NN Publication will be responsible to resend the print copy only and only if the print copy is lost or print copy is damaged in delivery / or there is delay more than 6 months.
According to India Post the delivery should be completed with in 1-3 weeks after dispatch of articles.
Privacy Policy-
NN Publicationl uses the email ids of authors and editors and readers for sending editorial or publication notification only, we do not reveal or sell the email ids to any other website or company.