INFLUENCE OF SALT STRESS ON GROWTH, LEAF PIGMENTATION, IONIC DISTRIBUTION AND METABOLITES ACCUMULATION OF TALINUM TRIANGULARE (JACQ.) WILLD. LEAFY VEGETABLES AS AFFECTED BY DIFFERENT CASSAVA PEEL LEVELS
DOI:
https://doi.org/10.53555/nnfaes.v8i9.1419Keywords:
cassava peel, leaf pigmentation, metabolites, salt stress, Talinum triangureAbstract
Talinum triangulare Jacq. (Water leaf) is a leafy vegetable eaten in most countries in Africa in preparation of soups to enrich the starchy main dishes due to its tastes, medicinal uses such as antiinflammation, anti-fungal, anti-bacterial properties. Impact of four levels of cassava peel (0, 9, 12 and 15 t ha-1) in water leaf in terms of leaf pigment, nutrients, matabolites, antioxidant compounds, minerals, growth and yield under salt stress (0, 50, 100 and 200 mM NaCl) were investigated. Results show that salinity decreased growth parameters, total photosynthetic pigments, ascorbic acid and yield components accompanied by increases in sodium content, carbohydrate, soluble proteins, tannins total phenolic content and flavonoid in the leaf of water leaf. On the other hand, all applied treatments cassava peel caused significant increases in most parameters under investigation as growth parameters, minerals content, all components of photosynthetic pigments, and leaf yield, metabolites. It is obvious that cassava peel treatment at 15 t ha-1 was the most pronounced treatment either under non saline conditions or under salt stress conditions. Increased osmolyte and metabolite accumulation, and redox components in CP supplemented plants regulated the NaCl tolerance by further strengthening the antioxidant mechanisms. The increasing CP supplementation regulates salt tolerance in T. triangulare through modulations in the metabolism of antioxidants, osmolytes and metabolites.
References
Abreu, C.A., et al., 1995. Comparação de métodos de análise para avaliar a disponibilidade de metais emsolos. Revista Brasileira de Ciência do Solo, Campinas 19, 463-468.
Abogadallah, G.M., 2010. Antioxidative defense under salt stress. Plant Signaling and Behavior, 5, 369– 374. PMID: 20118663
Ahanger, M.A., Alyemeni, M.N., Wijaya, L., Alamri, S.A., Alam, P., Ashraf, M., Ahmad, P., 2018. Potential of exogenously sourced kinetin in protecting Solanum lycopersicum from NaCl-induced oxidative stress through up-regulation of the antioxidant system, ascorbate-glutathione cycle and glyoxalase system. PLoS One. 13 (9), e0202175. https://doi.org/10.1371/journal. pone.0202175
Aja, P.M., Okaka, A.N., Onu, P.N., Ibiam, U. and Urako, A. J., 2010. Phytochemical Composition of Talinium Triangulare (water leaf) Leaves. Pakistan Journal of Nutrition. 9 (6), 527-530.
Alam, M.A., Juraimi, A.S., Rafii, M.Y., Hamid, A.A., Aslani, F., Alam, M.Z., 2015. Effects of Salinity and Salinity-induced Augmented Bioactive Compounds in Purslane (Portulaca oleracea L.) for Possible Economical Use. Food Chem. 169, 439–447.
Amirjani, M.R., 2010. Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Amer. J. Plant Physiol. 5, 350-360.
Ayoola, O.T., Saka, J.O. and Lawal, B.O., 2009. Resources Efficiency in Dry season Vegetable Production. International Journal of Vegetable science. 15 (2), 86-95. DOI: 10.1080/193.
Babu, M.A., Singh, D. and Gothandam, K.M., 2011. Effect of Salt Stress on Expression of Carotenoid Pathway Genes in Tomato. Journal of Stress Physiology & Biochemistry, 7, 87-94
Benito, B., Haro, R., Amtmann, A., Cuin, T.A., Dreyer, I., 2014. The twins K+ and Na+ in plants. J. Plant Physiol. 171, 723–731.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254,
Burhan, A.K., AL-Taey, D.K., 2018. Effect of Potassium humate, humic acid, and compost of rice wastes in the growth and yield of two cultivars of dill under salt stress conditions.Adv Nat Appl Sci. 12 (11), 1-6
Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C., 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food Drug Analysis 10, 178-182. https://doi.org/10.38212/2224-6614.2748
Dubois, M., Gilles, K.A, Hamilton, J.K., Rebers, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350-356.
Dumas, Y., Dadomo, M., Di Lucca, G. and Grolier, P., 2003. Effects of Environmental Factors and Agricultural Techniques on Antioxidant Content of Tomatoes. Journal of the Science of Food and Agriculture 83, 369-382. https://doi.org/10.1002/jsfa.1370
Ezekwe, C.I., Chidinma, R.U., and Okechuckwu, P.C.U., 2013. The Effects of methanol Extract of Talinum triangulare (water leaf) on the Hematolog and some Live Parameters of Experimental Rats. Global Journal of Biotechnology and Biochemistry 8 (2), 51-60.
Gahler, S., Otto, K. and Bohm, V., 2003. Alterations of Vitamin C, Total Phenolics, and Antioxidant Capacity as Affected by Processing Tomatoes to Different Products. Journal of Agricultural and Food Chemistry 51, 7962-7968. https://doi.org/10.1021/jf034743q
Gill, S.S., Tuteja, N., 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48, 909–930. https://doi.org/10.1016/j.plaphy.2010. 08.016 PMID: 20870416
Gossett, D.R., Millhollon, E.P. and Lucas, M.C., 1994. Antioxidant Response to NaCI Stress in Salt Tolerant and Salt-Sensitive Cultivars of Cotton. Crop Science 34, 706-714. https://doi.org/10.2135/cropsci1994.0011183X003400030020x
Grace, S. C., and Logan, B.A. 2000. Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Philos Trans R. Soc. B. v. 355, p. 1499-1510.
Hanafy, Ahmed, A.H., Higazy, M.A., El-Shafey, Y.H. and Moussa, S.F., 2002. Effect of Salinity, Silicon and Proline on the Growth, Yield and Chemical Composition of Wheat Plant. Proceedings of the 2nd Congress Recent Technology Agriculture, Cairo University, 28-30 October 2002, 965-978.
Hoagland, D.R., Arnon, D.I., 1950. The Water-Culture Method for Growing Plants without Soil; California Agricultural Experiment Station: Berkeley, CA, USA.
IFOAM., 2005. Norms of IFOAM basic standards for organic production and processing IFOAM, Germany. http://www.ifoam.org. Accessed 31st December 2011
Iqbal, N., Umar, S., Khan, N.A., 2015. Nitrogen availability regulates proline and ethylene production and alleviates salinity stress in mustard (Brassica juncea). J Plant Physiol. 178, 84–91.
Irannejad, H. and Shahbazian, N., 2004. Field Crops Tolerance to Stress. University of Tehran Press, Tehran.
Jun Jin, W., Han, B.K., Yu, K.W., Kim, M.S., Chang, I., 2001. Antioxidant effects of Origanum majorana L. On superoxide anion radicals. Food. Chem, v. 75, p. 439-444. https://doi.org/10.1016/S0308-8146(01)00233-3
Kalu, C., Modugu, W.W. and Ubochi, I., 2009. Evaluation of soil waste management
policy in Benin Metroolis, Edo State, Nigeria. African Sci. 10, 1-7.
Kannaiyan, S., 2002. Biofertilizers for sustainable crop production. In: Biotechnology:
(Ed. S. Kannaiyan). Springer, Germany pp. 9-50.
Kargar, S.M.A. and S.J. Kareh, 2017. The evaluation of seed germination on 12 soybean cultivars under different salinity stress levels. Plant Breed Seed Sci. 73, 39-51.
Ksouri, R., Megdiche, W., Debez, A., Falleh, H.; Grignon, C., Abdelly, C., 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant. Physiol. Biochem. v. 45, p. 244-249. https://doi.org/10.1016/j.plaphy.2007.02.001
Kumar, N., Krishnamoorty, V., Nalina, L., Soorianathasundharam, K., 2002. A new factor for estimating total leaf area in banana. InfoMusa 11, 42-43.
Lichtenthaler, H.K., Wellburn, A.R., 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. In Biochemical Society Transactions, vol. 11, pp, 591–592
Malavolta, E., Vitti, G.C. and Oliveira, S.A., 1997. Avaliação do estado nutricional das plantas: princípios e aplicações. 2nd Edition, POTAFOS, Piracicaba, SP, 319 p.
Mannan, M.A., Karim, M.A., Haque, M.M., Khaliq, Q.A., Higuchi, H. and Nawata, E., 2013. Response of soybean to salinity: II. Growth and yield of some selected genotypes. Trop. Agr. Develop., 57 (1), 31-40.
Marigo, G., 1973. On a fractionation method and estimation of the phenolic compounds in plants. Analysis 2, 106-110.
Metwally, S.A., Khalid, K.A. and Abou-Leila, B.H., 2013. Effect of water regime on the growth, flower yield, essential oil and proline contents of Calendula officinalis. Nusantara Bioscience. 5 (2), 65-69. DIO:10.13057/nusbiosci/n050203.
Munns, R, Tester M., 2008. Mechanisms of salinity tolerance. Annual Review in Plant Biology, 59, 651–681.
Nagata, M. and Yamashita, I., 1992. Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo Gakkaishi 39, 925–928
Ogbo, F.C. and Odo, M.O., 2011. Potential of rice husk and cassava peel as carriers for bio-fertilizer production. Nigerian Journal of Biotechnology 23, 1-4.
Okalebo, J.R., Gathua, W.K., Woomer, P.L., 1993. Laboratory methods of soil and plant analysis: a working manual In Soil Biology and Fertility, Soil Science Society of East Africa, Kari, UNESCO-ROSTA Naïrobi, Kenya. 88 p.
Oluwole, S.O., Ogun, M.L. and Balogun, O.A., 2018. Effects of different watering regimes on the growth Talinum triangulare Jacq. Journal of Research and Review in Science. 5, 14-23
Onwudike, S.U., Asawalam, D.O. and Ano, A.O., 2016. Influence of Agro-waste on soil properties, growth and yield of Maize (Zea mays L.) in an acid utisol of Oerri, Imo state. Nigerian Journal of Soil Science 26, 279-294.
Parvez, S., Abbas, G., Shahid, M., Amjad, M., Hussain, M., Asad, S.A., Imran, M., Naeem, M.A., 2020. Effect of salinity on physiological, biochemical and photostabilizing attributes of two genotypes of quinoa (Chenopodium quinoa Willd.) exposed to arsenic stress. Ecotoxicol. Environ. Saf. 187, 109814.
Pauwels, J.M., Van Ranst, E., Verloo, M. and Mvondo, Z.A., 1992. Analysis Methods of Major Plants Elements. Pedology Laboratory Manual: Methods of Plants and Soil Analysis. Stock Management Equipment of Worms and Chemical Equipment. Publica Agricol. 28, AGCD, Brussels.
Selmar, D., Kleinwachter, M., 2013. Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants. Industrial Crop Production 42, 558–566.
Shabala, S., Pottosin, I., 2014. Regulation of potassium transport in plants under hostile conditions: Implications for abiotic and biotic stress tolerance. Physiol. Plant. 151, 257–279.
Shad, M.A., Nawaz, H., Rehman, T. and Ahmad, H.B., 2012. “Optimization of extraction efficiency of tannins from Cichorium intybus L.: application of response surface methodology,” Journal of Medicinal Plants Research, vol. 6, pp. 4467–4474.
Singh, M., Kumar, J., Singh, S., Singh, D.V., Prasad, S., 2015. Roles of Osmoprotectants in Improving Salinity and Drought Tolerance in Plants: A Review. Rev. Environ. Sci. Bio/Technol. 14.
Sutrisno, S., Yusnawan, E., 2018. Effect of manure and inorganic fertilizers on vegetative, generative characteristics, nutrient, and secondary metabolite contents of mungbean. Biosaintifika 10, 56-65. https://journal.unnes. ac.id/nju/index.php/biosaintifika/article/ view/12716
Taffouo, V.D., Djiotie, N.L., Kenne, M., Din, N., Priso, J.R., Dibong, S., Akoa, A., 2008. Effect of salt stress on physiological and agronomic characteristics of three tropical cucurbit species. Journal of Applied Bioscience 10, 434-441.
Taffouo, V.D., Wamba, F.O., Youmbi, E., Nono, G.N., Amougou, A., 2010. Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranea L. verdc.) landraces grown under saline conditions. International Journal of Botany, 6 (1), 53-58.
Taleisnik, E., Peyrano, G., Arias, C., 1997. Response of Chloris gayana cultivars to salinity. 1. Germination and early vegetative growth. Trop. Grassl. 31, 232-240.
Wahid, A., Ghazanfar, A., 2006. Possible Involvement of Some Secondary Metabolites in Salt Tolerance of Sugarcane. J. Plant Physiol. 163, 723–730.
Walkley, A., Black, I.A., 1934. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 37, 29-38.
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