Prevalence and Molecular Characterization of Shiga Toxin Producing E. coli Isolated from Some Locally Produced Beef Products
DOI:
https://doi.org/10.53555/nnmhs.v2i5.630Keywords:
STEC, Serovars, Genotypes, beef productsAbstract
Shiga toxin-producing Escherichia coli (STEC) may cause severe gastrointestinal and systematic diseases in humans that result from consumption of meat products. STEC were isolated from some beef products on Trypticase Soya Broth and Sorbitol MacConkey agar supplemented with cefixime and tellurite supplements and were biochemically identified . Further identifications were performed including Vero cells cytotoxicity assay and PCR technique for specific VT1/VT2 and eae genes. It was obvious from the obtained resuts that the incidance of shiga-toxin producing E.coli were 22%, 12%, 10%, 16% and 2% in minced meat, beef burger, beef sausage, beef kofta and beef luncheon, respectively. The serovars of shiga-toxin producing E.coli isolated from the examined meat samples were O111, O26, O103, O119 ,O128 ,O86, O45 ,O146 ,O119 and O121. E.coli O111, O26, O103, O91, O86 and O119 that proved to have Stx1 and Stx2 genes. E.coli O128 and O121 had only Stx1, while E.coli O146 had only Stx2.Concerning the eae gene responsible for the attaching and effacing lesions, E. coli O111 and O26 isolates proved to possess such gene .In conclusion beef beef products constitute an important reservoir of STEC infection to man and it was decleared that PCR technique is the most rapid, sensitive and efficient approach for detection of STEC in beef products
References
Abd –EL-All, A. M. (2005): Prevalence of shiga-toxin producing E. coli in farm animals with reference to environmental sanitation. Ph.D. Thesis, Fac. Vet. Med. Zagazig Univ. Egypt.
Abd-EL-Lateif, G. S. (2003): Polymerase chain reaction and electrophoretic pattern of certain types of Eschershia coli. M.V.SC. Fac.Vet. Med. Alex. Univ.Egypt.
Alfredo C.; Stefano M, Hubert and Eric O. (2005): Enterohaemorrhagic Escherichia coliemerging issues on virulence and mode of transmission.Vet.Res.,36:289-311.
Bettleheim, K.A .(2000): Role of non-O157 VTEC. J. Appl. Microbiol. Symp. Suppl;88:38S-050S.
Bhong, C.D.; Brahmbhatt, M.N.; Joshi, C.G. and Rank, D.N.(2008): Detection of virulence determinants by real time PCR in E. coli isolated from mutton. Meat Science, 80 : 1129–1131.
Bollinger, L.M. (2004): Effects of season and animal factors on prevalence of shiga toxinproducing E. coli in beef cattle. MS.V.Sc., Univ. of Nevada, Reno. United States.
Byomi, A.M.; Radwan, G.s. and Bahy El-Gamal G. Abd El-Aal (2001): Chacterization of Shiga toxin producing E.coli (stx1) from bovine by colony blot hybridization and its public health importance. Egypt. J. Biomed. Sci., 7:9-16.
Dambrosio A.A., Lorusso V.V., Quaglia, N.C., Virgilio G.S., Lucifora G.V. and Celano N.F. (2007): Escherichia coli O26 in minced beef: prevalence, characterization and antimicrobial resistance pattern. Inter J. Food Microbiol. 118: 218-222.
Fantelli, K.and Stephan, R. (2001): Prevalence and characteristics of Shigatoxin-producing Escherichia coli and Listeria monocytogenes strains isolated from minced meat in Switzerland. International Journal of Food Microbiology, 70 : 63–69
Gyles, C.L. (2007) Shiga toxin-producing Escherichia coli: anoverview. J Anim Sci 85, E45–E62.
Hassan, S.A. and Elmalt, L.M (2008): Informally raw milk and Kareish cheese investigation on the occurance of toxigenic Escherichia coli in Qena city, Egypt with emphasis on molecular characterization. Ass. Univ. Bull. Environ. Res. 11(2): 35-42.
Hussein . H. S.,(2007): Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their products. J. Anim. Sci. 2007. 85(E. Suppl.):E63–E72 doi:10.2527/jas.2006-421.
Hussein, H. S., and L. M. Bollinger.( 2005): Prevalence of Shiga toxinproducing Escherichia coli in beef. Meat Sci. 71:676–689.
Hornitzky, M.A.; Vanselow, B.A.; Walker, K.; Bettelheim, K.A.; Corney, B.; Gill, P.;Baily, G. and Djordjeevic, S.P.(2002): Virulence properties and serotypes of shiga toxin-producing E. coli from healtrhy Australian cattle. Appl. Environm. Microbiol. 68:6439-6445.
Jenkins, C.; Pearce, M.C.; Chart, H.; Cheasty, T.; Willshaw, G.A.; Gunn, G.J.; Dougan, G.; Smith, H.R.; Synge, B.AS. and Frankel, G. (2002): An eight-month study of a population of verocytotoxin-producing Escherichia coli(VTEC) in a Scottish cattle herd. J. Apll. Microbiol., 93: 944-953.
Karmali, M.A. (1989): Infection by Verocytotoxin-producing Escherichia coli. Clinical Microbiology .Rev. 2:15- 38.
Konowalchuk, J.; Speirs, J. I. and Stavric, S. (1977). Vero response to a cytotoxin of Escherichia coli. Infect. Immun. 18:775-779.
Kok, T.; Worswich, D. and Gowans, E. (1996): Some serological techniques for microbial and viral infections. In Practical Medical Microbiology (Collee, J.; Fraser, A.; Marmion, B. and Simmons, A., eds.), 14th ed., Edinburgh, Churchill
livingstone, UK.Lee,G.Y.; Jang,H.I.; Hwang,I.G. and Rhee,M.S.(2009): Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. International Journal of Food Microbiology, 134 : 196–200.
Lindgren, S.W., Melton, A.R. and O’Brien, A.D. (1993):Virulence of enterohemorrhagic Escherichia coli O91:H21clinical isolates in an orally infected mouse model. Infect Immun 61, 3832–3842..
Mohammed, M. E.M. (2002): Epidemiological studies on some zoonotic bacteria of foodborne diseases. Ph.D. Thesis, Fac. Vet. Med. Zagazig Univ. Egypt.
Monaghan, A., Byrne, B., Fanning, S., Sweeney, T., McDowell,D. and Bolton, D.J. (2011) Serotypes and virulence profiles of non-O157 Shiga toxin-producing Escherichia coli isolates from bovine farms. Appl Environ Microbiol 77,8662–8668.
Muniesa, M., Blanco, J.E., De Simon, M., Serra-Moreno, R.,Blanch, A.R. and Jofre, J. (2004) Diversity of stx2 converting bacteriophages induced from Shiga-toxin producing Escherichia coli strains isolated from cattle.Microbiology 150, 2959–2971.
Paton, J.C. and Paton, A. W. (1998): Pathogenesis and diagnosis of Shiga- toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11(3):450-479.
Pennington, H. (2010) Escherichia coli O157. Lancet, 376,1428–1435.
Riley, L.W. (1987): The epidemiological clinical and microbiological features of hemorrhagic colitis. Annual Review of Microbiol., 41: 383-407.
Saleh, S.K. (2001): Prevalence of Enterohemorrhagic E.coli in some meat products. J. Egypt. Vet. Med. Ass., 61(4): 173-178.
Sambrook, J.; Fritsch, E. F. and Maniatis, T. (1989): Molecular cloning: Laboratory Manual. 2nd Edition, Cold spring, Harbor, New York, USA.
Sayed, A.M.; Abou El- Alla, A.A.; Abd El-Hafez, M.M.; Hussein, A.A.; and Hassanien, Z.A. (2001): Prevalence of Escherichia coli with special reference to E.coliO157 : H7 in some retail meat products in Assiut Governorate. Assiut. Vet.
Med. J., 45(90): 146-155.
Shima, K; Wu, Y ; Sugimoto,N; Asakura,M; Nishimura,K and Yamasaki, S (2006): Comparison of a PCR-Restriction Fragment Length Polymorphism(PCRRFLP) Assay to Pulsed-Field Gel Electrophoresis To Determine the Effect of Repeated Subculture and Prolonged Storage on RFLP Patterns of Shiga Toxin Producing Escherichia coli O157:H7._ J. CLinc. Mcrbiol., 44(11): 3963–3968
Smith, H. R., and Scotland, S. M. (1988): Vero cytotoxin-producing strains of Escherichia coli. J. Med. Microbiol. 26:77-85.
Samadpour, M., J. E. Ongerth, J. Liston, N. Tran, D. Nguyen, T. S.Whittman, R. A. Wilson, and P. I. Tarr.(1994).:Occurrence of Shiga-like toxin-producing Escherichia coli in retail fresh seafood,beef, lamb, pork, and poultry from grocery stores in Seattle,Washington. Appl. Environ. Microbiol. 60:1038–1040.
Xiaodong, X.(2010): Pathogenic E.coli in retail meats. Dissertation submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Doctor of Philosophy 2010
Zhao, C., B. Ge, J. De Villena, R. Sudler, E. Yeh, S. Zhao, D. G. White,D. Wagner, and J. Meng. (2001). Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken,turkey, pork, and beef from the Greater Washington, DC area.Appl. Environ. Microbiol. 67:5431–5436.
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