ASSESSMENT OF IL-2, IL-10, TNF AND SELENIUM LEVELS IN SICKLE CELL ANAEMIA INDIVIDUALS IN THEIR STEADY STATE ATTENDING NAUTH, NNEWI IN SOUTHEASTERN NIGERIA.
DOI:
https://doi.org/10.61841/mh8h3p49Abstract
Background: Sickle-cell anemia (SCA) results from a point mutation, in which an adenine nucleobase in the sixth codon of the globin gene is replaced by a thymine (GAG GTG). The immune-inflammatory system is a complex network of cells and humoral elements that includes many cytokines. Cytokines are peptides used by cells for intercellular communication and for controlling the inner environment of the cells in which they operate. They are produced by cell types that have important roles in the immune response, inflammation, haemopoiesis, healing and systemic response to injury.
Aim: The aim of this study was to assess serum levels of IL-2, IL-10 &TNF in sickle cell disease anemia subjects placed on selenium supplements in their steady state.
Methods: This is a cross- sectional study. A total of fifty aged matched participants were recruited for this study. 50 SCA subjects with sickle cell anemia (SS) were placed on selenium supplements 200mcg for 90 days.IL-2,1L-10& and TNF were measured using enzyme linked immunosorbent assay (ELISA) method.
Results: The result showed that the mean serum level of IL-2, (119.0621.68), significantly reduced while IL-10 and TNF (119.5919.20 and 16.232.83) significantly increased. Significance at (p0.05) after administration of selenium supplements while compared with the baseline values (125.0642.68, 116.4517.30 and 15.963.32) respectively.
Conclusion: SCA is a well-studied monogenetic disease with an established chronic inflammatory component. IL2 is a pro-inflammatory cytokine while both IL 10 and TNF are both anti-inflammatory cytokines. The intake of selenium supplement helped in suppressing the proinflammatory mediator while the protypical anti-inflammatory cytokine stepped their activities.
References
Steinberg, M.H.; Adewoye, A.H. Modifier genes and sickle cell anemia. Current Opinion.Hematology (2006) 13 :131–136.
Gille J, Swerlick R, Lawley T, Caughman S (2016). Differential regulation of vascular cell adhesion molecule gene transcription by TNF and IL 1a in dermal microvascular endothelial cells. Blood. 87:211-217.
Kato, G.J.; Steinberg, M.H.; Gladwin, M.T. Intravascular hemolysis and the pathophysiologyof sickle cell disease (2017). Journal of Clinical Investigation 1:12:750–760.
Liao C, Hardison RC, Kennett MJ, Carlson BA, Paulson RF, Prabhuk S (2018). Selenoproteins regulate stress erythroid progenitors and spleen microenvironment during stress erythropoiesis. Blood. 131:2568-2580.
Oluwadamilola AD, Akinremi TI, Adefisan MA, Olayiwola SD (2021). Knowledge, attitude and control practices of sickle cell disease among senior secondary students in Osun State Nigeria. Pan African Medical Journal. 38:350.
Pakbaz Z, Wur T (2014). Role of hemostatic system in SCD pathophysiology and potential therapeutics. Haematology Oncology Clinical North America Journal. 28:355-374.
Singhal A, Doherty JF, Raynes JG, McAdam KP, Thomas PW, Serjeant BE et al., (2013) Is there an acute phase response in Steady state sickle cell disease. Lancet 341 (8846): 651 653.
Steinberg MH (2019). Management of sickle cell disease. National England Journal of Medicine. 340:1021-1030.
Stuart J, Stone PC, Akinola NO, Gallimore JR, Pepsy MB (2014). Monitoring the acute phase response to vassocclusive crisis in sickle cell disease. Journal of Clinical Pathology 47(2):166-169.
Sun Y, Ma A, Li Y,Han X, Wang Q, Liang H (2012). Vitamin E supplementation protects erythrocyte membranes from oxidative stress in healthy Chinese middle-aged and elderly people. Nutritional Research. 32:328-332.
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