Evaluation the Activity of Antioxidants and Reproductive Performance for Allium cepa and Allium sativum Extracts on Males Rats Treated with Mycotoxins

Abstract

The present study has been designed to evaluate the beneficial effects of Allium cepa and Allium sativum extract upon Ochratoxin-induced hepatic toxicity, The study has been conducted on adult male rats during the period extended from February, 2017 to, July 2017. Fifty mature male Wister rats (aged 90 days and weighted 190±10 g),were divided in to Five randomly equal groups , the first served as negative control received only normal saline , the second as positive control received with contaminated with Asp. niger in diet  as a single dose(2.5 mg/kg b.w). third group received with contaminated with Asp. niger as a single dose then treated after 2 days with Allium cepa (250 mg /kg b.w.) respectively orally through stomach tube during 42 days. fourth group received contaminated with Asp. niger as a single dose then treated after 2 days with Allium sativum (250 mg /kg b.w.) respectively orally through stomach tube for 42 days.  Fifth group received contaminated with Asp. niger as a single dose then treated after 2 days with Allium cepa and Allium sativum together (250,250 mg /kg b.w.) respectively orally through stomach tube during 42 days.  males were anaesthetized (by injection i/p of 0.3ml ketamine + 0.1 ml of xylazine /kg b. w. ip), blood samples were obtained from heart in non-heparinized tubes, Blood serum samples were separated for assessment of GOT, GPT, concentration, and assessment testosterone, FSH in blood serum.  Under our experimental conditions, contaminated with Asp. niger resulted evidenced by statistically significant increase(p>0.05)  in the activities of GOT, GPT in group 2 of just Ochratoxin compared with the control group, while in the third and fourth groups showed  statistically significant decrease (p<0.05) in GOT, GPT level in Blood serum of contaminated with Asp. niger treated group compared with the control group, while in assessment of hormone shown in contaminated group showed decrease of this hormones as for as in Allium cepa and Allium sativum groups increase in hormones and in combination groups was very high significant in hormones levels. After histological analysis of the group contaminated with Asp. niger proved change in the, after the gavage of (Allium cepa and Allium sativum), and in testicular section was shown the effect of plants extract to repair of tissue. The results showed that there was an improvement in liver tissues after treatment with Allium cepa and Allium sativum. In present study we concluded to Allium cepa and Allium sativum together dose 250+250 mg/kg b.w. is better than dose 250mg/kg b.w. alone  depending on  results in recently study. we concluded that the administration of (Allium cepa and Allium sativum) together act as antifungal and antioxidant for contaminated with Asp. niger in males rats.