Production optimization of Alfa-amylase from Bacillus licheniformis
DOI:
https://doi.org/10.53555/nnpbs.v2i5.701Keywords:
Starch, α-amylase, optimization, thermophilic, alkaliphilicAbstract
Amylases are of great significance in present day biotechnology. They constitute 25% of the industrial market. To meet the industrial demand there is a need of production optimization of ?-amylase from the microbial source. In the present study, an attempt was made to isolate thermophilic bacterial strain producing thermophilic and alkaliphilic ?-amylase. Among 23 isolates, isolate K7 gave maximum production of ?- amylase, which was later identified as Bacillus licheniformis. The organism gave maximum production of ?- amylase in medium containing g/l (w/v) beef extract 3.0, peptone 5.0 and starch 1.0%. Starch (1.75%, w/v) and peptone (0.15%, w/v) were optimized to be best carbon and nitrogen sources for ?-amylase production from Bacillus licheniformis. Optimum production of the enzyme was observed when inoculated medium of pH 8.0 was incubated at 50°C for 48 h of incubation time.Further during optimization of reaction conditions, the enzyme gave maximum activity with 0.1 M Tris HCl buffer of pH 8.0 when incubated at a reaction temperature of 50°C for 10 min of incubation time. The enzyme showed high affinity towards starch (0.15%, w/v) as substrate. The thermophilic and alkaliphilic nature of the enzyme suggest its potential application in starch, detergent and textile industries.
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