HPLC Method Development and Validation for Simultaneous Estimation of Atropine Sulfate and Dexamethasone
DOI:
https://doi.org/10.53555/nnpbs.v2i3.715Keywords:
Atropine sulfate, Dexamethasone, HPLCAbstract
Analysis of pharmaceutical product is very important as it concerned with quality of life. The objective of present work is to develop sensitive, specific, reproducible, accurate method for determination of Atropine sulfate and Dexamethasone in its combined Liquid dosage form by HPLC method. In HPLC method for Atropine sulfate and Dexamethasone, chromatographic separation was carried out on Thermo Hypersil ,C18 column (250mm x 4.6mm i.d. 5?m) using mobile phase 0.02M Pottasium dihydrogen ortho Phosphate Buffer pH 4 : Methanol (75:25 v/v), flow rate 1.5 ml/min and detected at 210 nm. Linearity of Atropine sulfate and Dexamethasone found to be 25-75 and 2.5-7.5?g/ml respectively. Developed HPLC method was validated as per ICH guideline Q2 (R1), for its accuracy, precision, LOD & LOQ and the results were found to be satisfactory Developed and validated method was found to be simple, accurate, economical, robust and reproducible & method can be successfully applied for routine QC analysis.
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