Dual Role of RNase A and Metosartan: An Examination in invitro & in vivo Condition
DOI:
https://doi.org/10.53555/nnpbs.v5i5.742Keywords:
Metoprolol, Netosis, Uncompetitive inhibition, DNA adducts, Cyt P450Abstract
Metosartan consists of two components Metoprolol and telmisartan. Telmisartan inhibits RNase A in rat testes by uncompetitive inhibition where as Metoprolol forms DNA adducts and reduces sperm count. Metosartan decreases testes viability and sperm count was found to be high in metosartan treated rats compared to Metosartan +RNase A group. RNase A induces Netosis in both invivo and invitro condition in both RNase treated group and as well as RNase A+ Metosartan. Deep cold storage of mitochondria induces expression of cyt P450gene in testes mitochondria but not in sperm.
References
Komal Patel, Amit Patel, Jayant Dave, and Chaganbhai Patel (2012). Absorbance correction method for estimation of telmisartan and metoprolol succinate in combined tablet dosage forms. Pharm Methods. 2012 Jul-Dec; 3(2): 106–111.
Minoru Kurihara, Michio Ogawa, Toshiyuki Ohta, Eiji Kurokawa, Takeshi Kitahara, Goro Kosaki, Takehiko Watanabe, and Hiroshi Wada (1982). Purification and Immunological Characterization of Human Pancreatic Ribonuclease. Cancer research, 42, 4836-4841.
Eswari beeram(2017). Examination of testis for rnasea through column chromatography and immunofluorescence. Paripex - indian journal of research Volume-6, Issue-11, pp 715-716.
Giovanni Gotte, Alexander Mahmoud Helmy , Carmine Ercole , Roberta Spadaccini , Douglas V. Laurents , Massimo Donadelli , Delia Picone (2012).Double Domain Swapping in Bovine Seminal RNase: Formation of Distinct N- and C-swapped Tetramers and Multimers with Increasing Biological Activities PLOS ONE, Volume 7, Issue 10, e46804.
Ronald H. L. Li and Fern Tablin (2018). A Comparative Review of Neutrophil Extracellular Traps in Sepsis. Front Vet Sci. 2018; 5: 291.
Eswari Beeram (2018). Apoptosis of testis tissue by drug metosartan through activation of intrinsic pathway by the release of Cyt C from mitochondria International Journal of Chemical Studies; 6(1): 614-617
Eswari Beeram (2018). Inhibition Studies of RNase a in Invitro Condition by Drug Metosartan. Open Science Journal of Pharmacy and Pharmacology. Vol. 6, No. 4, pp. 34-38.
Eswari Beeram (2018). Chromatin integrity in testis by microscopy and drug inhibition studies of rnase a by uv visible spectroscopy. ejbps, Volume 5, Issue 01 232-237
el-Sayed MG, el-Sayed MT, Elazab Abd el S, Hafeiz MH, el-Komy AA, Hassan E (1998). Effects of some beta-adrenergic blockers on male fertility parameters in rats. Dtsch Tierarztl Wochenschr. 105 (1) : 10-2.
Rajalakshmi T. R., AravindhaBabu N., Shanmugam, K. T. and Masthan K. M. K. (2015). DNA adducts-chemical addons. J Pharm Bioallied Sci. (Suppl 1): S197–S199.
Robert M. Elder and Arthi Jayaraman (2012). Sequence-Specific Recognition of Cancer Drug-DNA Adducts by HMGB1a Repair Protein. Biophys J. 102(10): 2331–2338.
Amaral Sandra, Paula Mota, Ana Sofia Rodrigues, Luı´s Martins, Paulo J. Oliveira Joa˜o Ramalho-Santos (2008). Testicular aging involves Mitochondrial dysfunction as well as an increase in UCP2 levels and proton leak. FEBS Letters; 582: 4191–4196.
Hammadeh ME, al-Hasani S, Stieber M, Rosenbaum P, Küpker D, Diedrich K, Schmidt W (1996). The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. Hum Reprod. 11(11):2468-71.
John R. Hofstetter, Aiwu Zhang, Aimee R. Mayeda, Tim Guscar, John I. Nurnberger, and Debomoy K. Lahiri., Genomic DNA from Mice: A Comparison of Recovery Methods and Tissue Sources. Biochemical and Molecular Medicine; 1997, 62, 197–202.
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